Journal: The Journal of International Medical Research
Article Title: Expression of β1 adrenergic receptor in vascular anomalies in children
doi: 10.1177/03000605211047713
Figure Lengend Snippet: (a) Intramuscular hemangioma (6-year-old girl). (b) Same piece, large target (×40). Most of the tumor endothelial cells are positive for β1-adrenergic receptor (β1-AR). Cytoplasmic expression with moderate intensity was found in most tumor cells. Rare cells have nuclear expression and those with cytoplasmic expression show diffuse, heterogeneous granularity. Isolated endothelial cells lining adjacent vessels to hemangioma exhibited a distinct nuclear expression pattern. Outside of the hemangioma area, endothelial cells expressing β1-AR were extremely rare, but still present.
Article Snippet: The samples were incubated with a goat polyclonal primary antibody against human β1-AR (Novus Biologicals, Littleton, CO, USA) for 15 minutes prior to visualization using the Bond Polymer System Refine Detection System (Leica Biosystems).
Techniques: Expressing, Isolation